Efficacy | Evaluation Model | Evaluation Indicator | |
Whitening | Enzymatic Activity Assay | Tyrosinase Activity Assay | Inhibition rate of tyrosinase activity |
Measurement of intracellular tyrosinase activity in A375 human melanin cells | Inhibition rate of tyrosinase activity | ||
Measurement of intracellular melanin content in B16F10 mouse melanin cells | Melanin content | ||
Melanin Experiment | Measurement of intracellular tyrosinase activity in B16F10 mouse melanin cells | Inhibition rate of tyrosinase activity | |
Measurement of dendrite length and quantity in MNT-1 human melanin cells | Length and quantity of dendrites in melanocytes | ||
Length and quantity of dendrites in melanocytes | E.g TRP-1, TYR, etc. | ||
Measurement of intracellular melanin content in MNT-1 human melanin cells | Melanin content | ||
Fluorescent staining- Melanin transfer | Melanin transfer | ||
Antioxidant | DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay | DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay free radical scavenging rate | |
PTIO (2-phenyl- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) radical scavenging assay | PTIO (2-phenyl- 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) free radical scavenging rate | ||
Induction of oxidation stress model in HaCaT by UVB | Free radicals including ROS, SOD | ||
Anti-glycosylation | Bovine serum albumin (BSA) - Fructose Maillard reaction simulation | Inhibition rate of advanced glycation end products (AGEs) | |
High glucose-induced fibroblast glycation model | AGEs (CML), measurement of RAGE content, and measurement of glucose uptake | ||
Glycation model of natural aging in primary fibroblasts | AGEs (CML), measurement of RAGE content | ||
Contact Us
Quick Navigation